A novel anti-biofouling vibration-based mechanism with the combined effects of electric field and shear stress was reported (on PZT plates). This anti-fouling mechanism can also be activated by a vibrating micromachined Si/SiO2 membrane. In addition, it can incorporate the polymer coating, such as poly(ethylene glycol), unto PZT plates to further enhance the anti-fouling performance.
Another polymer (mono thiol polyglycidols) with branched structure was self assembled on the gold surface. Compared to poly(ethylene glycol), this hyperbranched polyglycidol is more resistant to protein adsorption with similar molecular weight. The hyperbranched polyglycidols are able to be modified to graft unto PDMS surface for reducing biofouling and regent loss. In addition, the carboxyl hyperbranched polyglycidols served as a cation exchanger can generate the functionality of PDMS device as an ion-exchange chromatographic column. This PDMS-based microfluidic device had shown promising to be a protein purification and separation tool.