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Parallel microfluidic arrays for surface plasmon resonance imaging

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Seminar Details
Presenter Name: 
Eric Ouellet
Faculty Supervisor(s): 
Eric.Lagally
Date: 
Wednesday, June 3, 2009 - 3:00pm
Location: 
Kaiser 3028
Seminar Abstract: 

Surface Plasmon Resonance Imaging (SPRI) is a label-free technique for the quantitation of binding affinities and concentrations for a wide variety of target molecules. Although SPRI is capable of determining binding constants for multiple ligands in parallel, current commercial instruments are limited to a single analyte stream and a limited number of ligand spots. Measurement of target concentration also requires the serial introduction of different target concentrations; such repeated experiments are conducted manually and are therefore time-intensive. Likewise, the equilibrium determination of concentration for known binding affinity requires long times due to diffusion-limited kinetics to a surface-immobilized ligand. We have developed an integrated microfluidic array using soft lithography techniques for SPRi-based detection and determined binding affinities of an antibody against human-alpha thrombin. The device consists of 264 element-addressable chambers isolated by microvalves. The resulting 700pL chamber volumes, combined with a serial dilution network for simultaneous interrogation of up to six different target concentrations, allows for further speeding detection times through reaction-rate limited kinetics. Finally, since each chamber can be addressed independently, this array is capable of interrogating binding events from up to 264 different immobilized ligands against multiple targets in a single experiment.

Presenter Biography: