Multiphoton microscopy (MPM) is a non-invasive, high-resolution imaging method for looking at thick biological tissues. We use a femtosecond laser to develop MPM systems which can acquire two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) simultaneously. The MPM system is used to image cells and extracellular matrix in turbid tissues.
Multiphoton microscopy is an emerging technique for high-resolution, non-invasive imaging of thick biological tissues. It utilizes femtosecond lasers to excite nonlinear optical contrast signals from tissues. By absorbing the energy from two excitation photons, a fluorescence photon or second-harmonic photon can be generated by tissues. The absorption of two photons simultaneously restricted the excitation to be from the focal point of an objective lens. Thus 3-dimentional high-resolution imaging can be obtained by scanning the focal point. Multiphoton microscopy can image single cells and cell-extracellular matrix interactions. It can be applied to image thick tissues to distinguish cancerous and normal tissues. Multiphoton microscopy is a promising method for early cancer detection.